论文部分内容阅读
为探讨由黄芪、当归、川芎等补肾活血药组成的中药健胎液防治胎儿宫内发育迟缓的作用机理，本研究采用被动吸烟法建立胎儿宫内发育迟缓（ＩＵＧＲ）动物模型，运用蛋白质ＳＤＳ—聚丙烯酰胺凝胶电泳法检测正常对照组、胎儿宫内发育迟缓组（ＩＵＧＲ组）、ＩＵＧＲ加健胎液组（用药组）孕鼠红细胞膜—骨架成份变化，并以胎鼠平均出生体重变化作疗效评价。结果与ＩＵＧＲ组相比，用药组胎鼠平均出生体重增加（Ｐ＜０．０１），孕鼠红细胞膜—骨架带３蛋白含量升高（Ｐ＜０．０１），高分子聚合物含量降低，而用药组以上各指标与正常组相比均无显著性差异（均Ｐ＞０．０５）。结论认为胎儿宫内发育迟缓的发生与胎盘微循环障碍密切相关，健胎液能够保护红细胞膜—骨架，维持红细胞正常形态、结构和功能，改善胎盘微循环，进而有防治胎儿宫内发育迟缓的作用。其保护机制可能与抗氧自由基对红细胞的损伤有关
To investigate the mechanism of prevention and treatment of fetal intrauterine growth retardation by Jianfei liquid, which consists of Bushen, Angelica sinensis, Rhizoma Chuanxiong, etc., this study adopted a passive smoking method to establish an intrauterine growth retardation (IUGR) animal model, using protein SDS- Polyacrylamide gel electrophoresis was used to detect changes in erythrocyte membrane-skeletal components of pregnant rats in the normal control group, intrauterine growth retardation group (IUGR group), IUGR plus Jiantaiye group (medication group), and the mean birth weight of fetal rats. For efficacy evaluation. Results Compared with the IUGR group, the average birth weight of the fetal rats in the treatment group increased (P<0.01), and the protein content of the erythrocyte membrane-skeleton 3 in pregnant rats increased (P<0.01), and the content of high molecular polymers decreased. There was no significant difference between the above indicators of the drug group and the normal group (all P>0.05). CONCLUSIONS: The occurrence of intrauterine growth retardation is closely related to placental microcirculatory disturbance. Kintaitonin can protect the red blood cell membrane-skeleton, maintain the normal morphology, structure and function of red blood cells, improve placental microcirculation, and then prevent and treat fetal intrauterine growth retardation. effect. Its protective mechanism may be related to the damage of red blood cells caused by antioxidant free radicals