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根据以往文献,用暗视野显微镜观察牙周袋或窦道分泌物中的细菌,直接观察活菌的形态,并以螺旋体占细菌总量的百分比可鉴别病灶是来自牙髓还是牙周,
According to the previous literature, the bacteria in the periodontal pocket or sinus secretions were observed with a dark-field microscope to directly observe the morphology of the viable bacteria. The percentage of spirochaetes in the total amount of bacteria was used to identify whether the lesions originated from the dental pulp or the periodontal tissue.