载脂蛋白E(apo-E)是血浆VLDL 的主要载脂蛋白之一,用等电聚焦电泳已鉴定出了app-E 的3个亚型:apo-E_2、apo-E_3和apo-E_4。人类常见的表现型有6种:E2/2、E3/3,E4/4、E2/3、E2/4和E3/4。最常用的鉴定载脂蛋白E 亚型的方法是用超速离心法分离出VLDL,再脱脂,作聚丙烯酰胺凝胶等也聚焦电冰。此法费时,且需超速离心机。本文用磷钨酸盐和氯化镁混合液沉淀VLDL, Apolipoprotein E (apo-E) is one of the major apolipoproteins of plasma VLDL. Three isoforms of app-E have been identified by isoelectric focusing electrophoresis: apo-E_2, apo-E_3 and apo-E_4. Six common human phenotypes are E2 / 2, E3 / 3, E4 / 4, E2 / 3, E2 / 4 and E3 / 4. The most commonly used method to identify apolipoprotein E subtypes is to isolate VLDL by ultracentrifugation, degrease, and focus polyacrylamide gels on ice. This method is time-consuming, and need to speed centrifuge. In this paper, a mixture of phosphotungstic acid and magnesium chloride precipitation VLDL,