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目的哨点监测2012-2013年新疆维吾尔自治区人民医院婴幼儿轮状病毒感染基因型情况,为轮状病毒(RV)疫苗的研制和使用提供依据。方法从新疆维吾尔自治区人民医院收集2012年8月至2013年10月5岁以下感染儿科和新生儿科住院腹泻和非腹泻患儿粪便样本203份,用ELISA方法检测轮状病毒,对阳性样本提取RV核酸,应用多重RT-PCR进行G型(VP7)和P型(VP4)基因分型。结果监测期间轮状病毒的检出率为33.5%(68/203),95.6%的RV阳性患儿≤24月龄,是RV感染的主要对象。对68份RV阳性样本进行基因分型,结果显示:感染儿科婴幼儿在2012年G型以G9(38.1%)为主要流行株,其次是G3(19.0%)、G2(14.3%)和G1(9.5%),混合感染达19.0%,1例未能分型;P型以P[8](71.4%)为主,其次是P[4](14.3%),混合感染占9.5%,1例未能分型;在2013年G型以G3(34.4%)为主,其次是G9(21.9%),G1和G2各占3.1%,混合感染型达31.3%,未能分型2例;P型同样以P[8](78.1%)为主,P[4]和P[6]各占3.1%,混合感染占15.6%。新生儿科新生儿以G9为主导(83.3%,10/12),1例为混合感染,1例未能分型;婴儿3例,2例G9,1例为G9+G3混合感染。新生儿P型以P[6]型为主(9/12),另有P[8]型2例,1例混合感染P[6+8];3例婴儿均为P[6+8]型混合感染。G/P组合基因型,感染儿科婴幼儿2012年主要以G9P[8]为主,其次为G3P[8],而2013年变为以G3P[8]为主,其次为G9P[8];新生儿科新生儿主要流行G9P[6],婴儿以混合感染为主。结论 RV仍然是本地婴幼儿腹泻的主要病原。2012-2013年婴幼儿RV G9P[8]型和G3P[8]型交替占主导流行。新生儿RV感染则以G9P[6]型为主要优势株。婴儿与新生儿间RV毒株的混合感染应引起高度关注。
The purpose of the sentinel surveillance of 2012-2013 People’s Hospital of Xinjiang Uygur Autonomous Region of infants and young children rotavirus infection genotypes, for the development of rotavirus (RV) vaccine and provide the basis. Methods Totally 203 stool samples from infants with diarrhea and non-diarrhea admitted to pediatric and neonatal departments under 5 years of age from August 2012 to October 2013 were collected from Xinjiang Uygur Autonomous Region People’s Hospital. Rotavirus was detected by ELISA and RV Nucleic acids were genotyped for type G (VP7) and type P (VP4) using multiplex RT-PCR. Results The detection rate of rotavirus was 33.5% (68/203) during monitoring, and 95.6% of RV-positive children ≤ 24 months old were the main targets of RV infection. The genotypes of 68 RV positive samples were analyzed. The results showed that the infants infected with pediatric GG were predominantly G9 (38.1%) in 2012, followed by G3 (19.0%), G2 (14.3%) and G1 9.5%), mixed infection was 19.0%, 1 case failed to type; P type was mainly P [8] (71.4%), followed by P [4] In 2013, G3 was predominantly G3 (34.4%), followed by G9 (21.9%), G1 and G2 each accounted for 3.1%, and mixed infection was 31.3% The same type of P [8] (78.1%), P [4] and P [6] each accounted for 3.1%, 15.6% mixed infection. Neonatal neonates were predominantly G9 (83.3%, 10/12). One patient was mixed infection and one patient failed to type. Three infants were infants, two were G9 and one was G9 + G3 mixed infection. Neonatal P type was predominantly P [6] (9/12), another was P [8], and 1 was mixed with P [6 + 8]; all 3 infants were P [6 + 8] Mixed infection. G / P combination genotypes, infants and young children infected mainly by G9P [8], followed by G3P [8] in 2012, G3P [8], followed by G9P [8] Pediatric neonates are predominantly G9P [6], with predominantly mixed infants. Conclusions RV is still the main cause of local diarrhea in infants and young children. 2012-2013 Infants and young children RV G9P [8] type and G3P [8] type alternately dominant. Neonatal RV infection is G9P [6] type as the main advantage strains. A mixed infection of RV strains between infants and newborns should be of great concern.