论文部分内容阅读
目的：进一步探讨图像分析术测定ＤＮＡ相对含量在肺癌中的预后意义。方法：随访８５例原发性非小细胞肺癌（ＮＳＣＬＣ）术后病例，图像分析术测定经Ｆｅｕｌｇｅｎ染色的肿瘤细胞核涂片的ＤＮＡ相对含量。Ｋａｐｌａｎ－Ｍｅｉｅｒ乘积限法用于生存分析。结果：本组病例中位随访时间为４７个月，３年、５年总体生存率分别为６６％和６１％；全部病例均为非整倍体，５ＣＥＲ均值为４５．９％±２３．６％，ＤＩ均值为３．０６±０．６３，５ＣＥＲ与ＤＩ之间有密切的相关性（ｒ＝０．８６，Ｐ＜０．００１），ＤＩ＜２．７９组和ＤＩ≥２．７９组病人的５年生存率分别为８０％和４３％，两者之间有显著差别（χ２＝１３．１，Ｐ＝０．００３）。讨论：图象分析术测定ＤＮＡ相对含量可作为判断ＮＳＣＬＣ预后的辅助指标。
Objective: To further explore the significance of image analysis in determining the relative content of DNA in lung cancer. METHODS: Eighty-five patients with primary non-small cell lung cancer (NSCLC) were followed up. Image analysis was performed to determine the relative DNA content of Fenugen stained tumor nuclei smears. The Kaplan-Meier product-limit method is used for survival analysis. Results: The median follow-up time was 47 months. The 3-year and 5-year overall survival rates were 66% and 61%, respectively. All cases were aneuploid and the mean 5CER was 45.9%±23.6. The mean value of DI and DI was 3.06±0.63. There was a close correlation between 5CER and DI (r=0.86, P<0.001), DI<2.79 and DI≥2.79. The 5-year survival rates of patients were 80% and 43%, respectively, with significant differences between them (χ2=13.1, P=0.003). Discussion: Image analysis to determine the relative amount of DNA can be used as an auxiliary indicator to determine the prognosis of NSCLC.