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用ＤＦＪ－１五码分级器对国产中型４９５Ａ－３３型柴油机颗粒物进行分级采样并对各不同粒径的柴油机颗粒物的提取物进行体外培养细胞微核实验。结果发现各粒径的颗粒物中以粒径＜１．１μｍ的颗粒物最多，占总颗粒物重量的４５．２％。且此部分颗粒提取物致体外细胞微核形成的能力最强，并随着颗粒粒径的增大微核出现率逐渐降低。这提示控制柴油机排出颗粒物的污染既要减少颗粒物质排出的总量，更要注重对小粒径颗粒物的过滤以有效降低柴油机排出颗粒物的毒性。
The DFJ-1 five-yard classifier was used to classify the domestic medium-sized 495A-33 diesel particulate matter and the micronucleus experiments were carried out in vitro on the diesel particulate matter of different particle size. As a result, it was found that the particles having a particle size of <1.1 μm were the most abundant among the particles of each particle size, accounting for 45.2% of the total weight of the particles. And this part of the particulate extract in vitro cell micronucleus formation ability of the strongest, and as the particle size increases the incidence of micronuclei decreased. This suggests that the control of diesel particulate matter emissions not only reduce the total amount of particulate matter emissions, but also pay attention to the small particle size of the particulate filter to effectively reduce the toxicity of diesel particulate matter.