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目的评估法国生物梅里埃VITEK2-COMPACT GN13药敏系统(VITEK2法)和纸片扩散法(K-B法)检测粘质沙雷菌对亚胺培南药敏结果的可靠性。方法本研究选取了50株金华市中心医院2014年6月至11月临床标本中分离出的非重复的粘质沙雷菌,分别采用VITEK2法、K-B法和微量肉汤稀释法测定其对亚胺培南的体外敏感性,以微量肉汤稀释法作为参考方法,评估VITEK2法、纸片扩散法与参考方法的分类一致率(CA%)。结果K-B法与参考方法的一致率为94.0%(47/50),仅有6.0%的小错误(MIE),未出现大错误(ME)和极大错误(VME);而VITEK2法与参考方法的一致率仅为48.0%(24/50),其中VITEK2法测定为敏感的菌株与参考方法的一致率达95.6%,但VITEK2法测定为耐药或中介的菌株与参考方法的一致率仅为7.4%,小错误率(MIE%)和大错误率(ME%)分别为55.6%和37.0%,未出现极大错误(VME)。结论 K-B法检测粘质沙雷菌对亚胺培南的药敏结果是比较可靠的;VITEK2法检测粘质沙雷菌对亚胺培南敏感的结果也是可靠的,但对亚胺培南非敏感结果的错误率(ME+MIE)高达到92.6%,实验室日常工作中若发现此类结果应采用K-B法或微量肉汤稀释法重新复核。
Objective To evaluate the reliability of the drug susceptibility test of marcelseria marcescens to imipenem by VITEK2-COMPACT GN13 and K-B method. Methods In this study, 50 non-repetitive S. marcescens isolated from clinical specimens of Jinhua Central Hospital between June and November 2014 were selected and tested by VITEK2 method, KB method and micro broth dilution method respectively In vitro sensitivity of amine penem, micro-broth dilution method as a reference method to assess the VITEK2 method, disk diffusion method and the reference method classification agreement rate (CA%). Results The agreement rates of KB method and reference method were 94.0% (47/50), only 6.0% of MIEs, no MEs and VMEs. However, VITEK2 method and reference method The concordance rate was only 48.0% (24/50). The consistent rate of the VITEK2 method was 95.6% of the reference method. However, the consistent rate of the VITEK2 method as resistant or intermediate strains was only 7.4%, MIE% and ME% were 55.6% and 37.0%, respectively. There were no major errors (VME). Conclusion KB method for the determination of serotonin in imipenem susceptibility results are more reliable; VITEK2 method for detecting serofreconsin sensitive to imipenem is also reliable, but insensitive to imipenem The results of the error rate (ME + MIE) up to 92.6%, laboratory work if found such results should be KB method or trace broth dilution method to review.