论文部分内容阅读
目的:调查市售红参须质量,制定红参须的质量标准。方法:收集不同地区市售红参须样品,采用性状观察,显微、薄层色谱鉴别,总灰分检查,高效液相色谱进行含量测定。结果:10批样品外观性状有差异;显微特征明显;薄层色谱中在与人参对照药材、人参皂苷Rb1、人参皂苷Re、人参皂苷Rf及人参皂苷Rg1相应的位置上显相同颜色的斑点;总灰分为2.50%～4.26%;人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1的线性范围分别为0.117～1.750,0.207～3.100,0.298～4.470μg,回收率分别为94.4%,95.2%,95.7%,RSD分别为1.99%,1.55%,1.54%,含量人参皂苷Rg1+人参皂苷Re为0.228%～0.666%,人参皂苷Rb1为0.312%～0.783%。结论:市售红参须质量差异较大,亟待制定红参须的质量标准。
Objective: To investigate the quality of red ginseng on the market and formulate the quality standard of ginseng. Methods: The red ginseng samples collected from different areas were collected, and their contents were determined by traits observation, microscopic and TLC identification, total ash test and high performance liquid chromatography. Results: The appearance of 10 batches of samples showed different microscopic features; the same color spots were observed in the thin layer chromatogram at the positions corresponding to the reference materials of ginseng, ginsenoside Rb1, ginsenoside Re, ginsenoside Rf and ginsenoside Rg1; The linear ranges of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 0.117 ~ 1.750,0.207 ~ 3.100,0.298 ~ 4.470μg, the recoveries were 94.4%, 95.2%, 95.7% , RSD were 1.99%, 1.55% and 1.54% respectively. The content of ginsenoside Rg1 + ginsenoside Re was 0.228% ~ 0.666%, ginsenoside Rb1 was 0.312% ~ 0.783%. Conclusion: The quality of commercially available red ginseng is quite different, so it is urgent to establish the quality standard of red ginseng.