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目的：建立一种简便快速的核酸浓缩回收新方法。方法：采用不同浓度的线性聚丙烯酰胺作为共沉淀载体，加入醋酸钠和无水乙醇后混匀离心，利用紫外检测和电泳方法确定对不同种类、大小和体积的核酸的回收率。结果：该方法可在１５ｍｉｎ内完成全部操作，适用于２０ｎｔ以上的单链和双链ＤＮＡ、ＲＮＡ的浓缩。对于０．１μｇ以上、大于４００ｂｐ的ＤＮＡ片段，回收率几乎达１００％。回收浓缩产物不影响后续的逆转录、ＰＣＲ、酶切和连接反应等操作。结论：所建立的方法适用于任何种类核酸的简便快速浓缩回收。
Objective: To establish a simple and rapid method for recovering nucleic acid. Methods: Different concentrations of linear polyacrylamide were used as coprecipitation carriers. After adding sodium acetate and absolute ethanol, the mixture was mixed and centrifuged. The recoveries of different kinds, sizes and volumes of nucleic acids were determined by UV detection and electrophoresis. Results: This method can be completed within 15min operation, suitable for more than 20nt single-stranded and double-stranded DNA, RNA concentration. For DNA fragments above 0.1 μg and above 400 bp, recovery was almost 100%. Recycling of concentrated products does not affect subsequent reverse transcription, PCR, digestion and ligation reactions. Conclusion: The established method is suitable for simple and rapid concentration and recovery of any kind of nucleic acid.