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采用聚合酶链反应(PCR)技术检测乙肝患者的血清HBV┐DNA,同时用ELISA法检测HBV各抗原抗体指标。结果显示,904例乙肝患者的HBeAg阳性率为37.9%,而HBV┐DNA阳性率达68.8%,血清HBVD┐NA阳性率明显高于HBeAg(P<0.05)。在HBeAg阴性病例中,HBV┐DNA阳性率达54.4%,其中有活动性肝病者阳性率为72.3%,肝病静止期仅19.9%,差异极显著(P<0.01)。提示PCR技术是了解乙肝病毒(HBV)复制情况的敏感方法,HBeAg阴性乙肝患者的肝损害因素主要与HBV在体内持续复制有关
Serum HBV DNA was detected by polymerase chain reaction (PCR) in patients with hepatitis B, and antibody indexes of each antigen of HBV were detected by ELISA. The results showed that the positive rate of HBeAg in 904 patients with hepatitis B was 37.9%, while the positive rate of HBV DNA was 68.8%. The positive rate of serum HBVDNA was significantly higher than that of HBeAg (P <0.05). In HBeAg-negative cases, the positive rate of HBV DNA was 54.4%, in which the positive rate of active liver disease was 72.3% and that of hepatic cirrhosis was only 19.9%, the difference was significant (P <0.01) . Tip PCR technology is a sensitive way to understand the replication of hepatitis B virus (HBV), liver damage in HBeAg-negative patients with hepatitis B and HBV in vivo sustained replication