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Objective:To evaluate anti-diabetic effect of Caulrpa kntillifera(C.lentillifera).Methods:The inhibitory effect of C.lentillifera extract on dipeptidyl peptidase-IV and a-glucosidase enzyme was measured in a cell free system.Then,interleukin-1βand interferon-γinduced cell death and insulin secretion were measured in rat insulinoma(RIN)cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit,respectively.Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting,using 3T3-Ll adipocytes.Results:C.lentillifera extract significantly decreased dipeptidyl peptidase-IV and a-glucosidase enzyme activities,and effectively inhibited cell death and iNOS expression in interleukin-1βand interfcron-γinduced RIK cells.Furthermore,C.lntillifera extract significantly enhanced insulin secretion in RTN cells and glucose transporter expression and glucose uptake in 3T3-L1adipocytes.Conclusions:Thus,our results suggest that C.lentillifera could be used as a potential antidiabetic agenl.
Objective: To evaluate anti-diabetic effect of Caullapa kntillifera (C. lentillifera). Methods: The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV and a-glucosidase enzyme was measured in a cell free system. Chen, interleukin-1β and interferon-γinduced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively.Glucose uptake and glucose transporter expression were measured by fluorometry and western blotting, using 3T3-L1 adipocytes. Results: C. lentillifera extract significantly decreased dipeptidyl peptidase-IV and a-glucosidase enzyme activities, and effectively inhibited cell death and iNOS expression in interleukin-1 β and interfcron-γinduced RIK cells.Furthermore, C.lntillifera extract significantly enhanced insulin secretion in RTN cells and glucose transporter expression and glucose uptake in 3T3-L1adipocytes. Conclusions: Thus, our results suggest that C.len tillifera could be used as a potential antidiabetic agenl.