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本文研究了固定化里氏木霉（TrichodermareeseiRutC30）在不同碳源条件下合成纤维素酶的时间进程及酶系组成特征。试验结果表明，碳源的性质对纤维素酶复合体系的组成有明显影响。在以纸浆为碳源制备的酶液中C_1／FPA和C_x／FPA的比值较大，分别为39．32和107．41，而CB／FPA的比值较小（0．08）；在以淀粉水解液为碳源所制备的纤维素酶液中C_1／FPA和C_x／FPA的比值较小，分别为28.54和66．44，但CB／FPA的比值明显较大（0．14）。当采用复合碳源（淀粉水解液和纸浆）时，发酵液中C_1、C_x：和纤维二糖酶的三种组分的活力均可达到较高水平，C_1／FPA和C_x／FPA分别可达36．2和95．10，与单独采用可溶性碳源时相比有了明显提高，CB／FPA也可达到0．12，与碳源为纸浆时相比提高了50％。本研究结果在走向调节纤维素酶复合体系的构成，对进一步提高酶制剂的实际应用效能方面将具有重要的参考价值。
In this paper, we studied the time course and enzyme composition of immobilized Trichoderma reeseiRutC30 under different carbon sources. The experimental results show that the nature of the carbon source has a significant effect on the composition of the cellulase complex system. The ratio of C 1 / FPA to Cx / FPA in the enzyme solution prepared from pulp as carbon source was 39.32 and 107.41, respectively, while the ratio of CB / FPA was smaller (0.08) The ratio of C 1 / FPA and C_x / FPA was 28.54 and 66.44, respectively, but the ratio of CB / FPA was significantly larger (0.14). When the composite carbon source (starch hydrolyzate and pulp) was used, the activities of C_1, C_x: and cellobiase in the fermentation broth reached a high level, and C_1 / FPA and C_x / FPA were up to 36.2 and 95.10, which are obviously improved compared with that of soluble carbon source alone. The CB / FPA can reach 0.12, which is 50% higher than that when the carbon source is pulp. The results of this study will play an important reference value in regulating the composition of cellulase complex system and further improving the practical application efficiency of enzyme preparation.