【摘 要】
：
Ethyl ferulate (EF) is abundant in Rhizoma Chuanxiong and grains (e.g.,rice and maize) and possesses antioxidative,antiapoptotic,antirheumatic,and anti-inflammatory properties.However,its effect on lipopolysaccharide (LPS)-induced acute lung injury (ALI)
【机 构】
：
Wuxi School of Medicine,Jiangnan University,Wuxi 214122,China;School of Food Science and Technology,
论文部分内容阅读
Ethyl ferulate (EF) is abundant in Rhizoma Chuanxiong and grains (e.g.,rice and maize) and possesses antioxidative,antiapoptotic,antirheumatic,and anti-inflammatory properties.However,its effect on lipopolysaccharide (LPS)-induced acute lung injury (ALI) is still unknown.In the present study,we found that EF significantly alleviated LPS-induced pathological damage and neutrophil infiltration and inhibited the gene expression of proinflammatory cytokines (TNF-α,IL-1β,and IL-6) in murine lung tissues.Moreover,EF reduced the gene expression of TNF-α,IL-1β,IL-6,and iNOS and decreased the production of NO in LPS-stimulated RAW264.7 cells and BMDMs.Mechanistic experiments revealed that EF prominently activated the AMPK/Nrf2 pathway and promoted Nrf2 nuclear translocation.AMPK inhibition (Compound C) and Nrf2 inhibition (ML385) abolished the beneficial effect of EF on the inflammatory response.Furthermore,the protective effect of EF on LPS-induced ALl was not observed in Nrf2 knockout mice.Taken together,the results of our study suggest that EF ameliorates LPS-induced ALl in an AMPK/Nrf2-dependent manner.These findings provide a foundation for developing EF as a new anti-inflammatory agent for LPS-induced ALI/ARDS therapy.
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