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定位是微生态学研究的重要内容之一，细菌的生长与其定植的部位密切相关。本研究的目的是为了探索微生态制剂研究中急性毒性试验的给药途径对试验结果的影响。采用从无感染烧伤创面分离的孔氏葡萄球菌和金黄色葡萄球菌标准菌株ＡＴＣＣ２５９２３分别接种在腹腔、静脉和皮内，观察７２小时。其间观察小鼠的外表、行为、饮食和死亡时间，并计算死亡率；取心、肝和脾进行细菌培养。结果皮内接种两种细菌和静脉注射孔氏葡萄球菌均无动物死亡，其余各组均有较高死亡率。各种接种途径中以静脉注射孔氏葡萄球菌后内脏器官细菌培养阳性率最低（１２５％），而以静脉注射金黄色葡萄球菌后内脏器官细菌培养阳性率最高（１００％）。我们认为，在进行微生态制剂的急性毒性试验时，应根据细菌的生物学特性来确定接种途径，其中至少要包含一种该受试制剂推荐临床研究的接种途径显得尤为重要。
Positioning is one of the important contents of micro-ecology research. The growth of bacteria is closely related to the site of its colonization. The purpose of this study was to explore the impact of the route of administration of the acute toxicity test in the study of probiotics on the test results. Staphylococcus aureus and Staphylococcus aureus standard strain ATCC25923 isolated from non-infected burn wounds were inoculated intraperitoneally, intravenously and intradermally for 72 hours respectively. During the observation of mice appearance, behavior, diet and time of death, and calculate the mortality rate; coring, liver and spleen for bacterial culture. Results There was no animal death in both intradermal inoculation and Staphylococcus aureus. All the other groups had higher mortality. The positive rate of bacterial culture in internal organs after intravenous injection of Staphylococcus aureus was the lowest (125%) in all routes of vaccination, while the positive rate of bacterial culture in internal organs was the highest (100%) after intravenous injection of Staphylococcus aureus. In our opinion, when carrying out the acute toxicity test of probiotics, the route of vaccination should be determined based on the biological characteristics of the bacteria, and at least it is important to include at least one route of vaccination for recommending the clinical study of the test preparation.