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目的探讨原因不明反复流产妇女外周血单个核细胞分泌Th细胞因子。方法采用细胞分离,混合淋巴细胞培养实验和用酶联免疫吸附法检测20例反复自然流产患者血液中的分泌Th细胞因子水平,并以20例正常健康早孕组为对照。结果反复自然流产患者IFN-γ高于对照组,IL-10低于对照组数据经统计学比较差异有统计学意义(P<0.05)。结论反复自然流产患者的Th的分泌的IFN-γ,IL-10细胞因子失衡可能是反复自然流产的原因之一。
Objective To investigate the secretion of Th cytokines by peripheral blood mononuclear cells in women with unexplained repeated abortion. Methods Secretion of Th cytokines in blood of 20 patients with recurrent spontaneous abortion was detected by cell separation, mixed lymphocyte culture and enzyme-linked immunosorbent assay (ELISA). Twenty normal healthy pregnancy groups were used as controls. Results IFN-γ in patients with recurrent spontaneous abortion was higher than that in control group. The data of IL-10 lower than control group showed statistically significant difference (P <0.05). Conclusion The imbalance of IFN-γ and IL-10 secreted by Th in patients with recurrent spontaneous abortion may be one of the causes of recurrent spontaneous abortion.