目的：为了探讨免疫介质ＬＰＳ对胚胎胰岛分泌ＩＬ６以及对胰岛功能的影响，以便为研究免疫系统与内分泌系统的内在联系提供依据。方法：采用常规消化方法分离人胚胎胰岛并对其进行原代培养，加入浓度为１０ｍｇ／ＬＬＰＳ，经不同时相收获上清，测定ＩＬ６、胰岛素、胰高血糖素含量和ＩＬ６ＭｃＡｂ中和试验，对ＬＰＳ刺激和对照组胰岛ＲＮＡ进行ＩＬ６ＲＮＡ斑点杂交。结果：①人胚胎胰岛经ＬＰＳ刺激分泌ＩＬ６的能力明显加强，在体外培养８ｄ，ＩＬ６含量达高峰，为对照组的１４５倍；②人胚胎胰岛在换新鲜培养液后用ＬＰＳ二次刺激，仍可继续分泌高效价的ＩＬ６，高峰时相为对照组的２５倍；③ＩＬ６ＭｃＡｂ对胰岛培养上清中ＩＬ６有很强的中和效应；④斑点杂交结果显示ＬＰＳ刺激后胰岛ＲＮＡ中ＩＬ６ｍＲＮＡ含量明显高于对照组；⑤人胚胎胰岛受ＬＰＳ刺激后胰岛素分泌能力加强，胰高血糖素分泌能力明显降低。结论：免疫介质ＬＰＳ可能影响胰岛的分泌功能及产生ＩＬ６的能力 Objective: In order to investigate the effect of immune mediator LPS on the secretion of IL6 by embryonic islets and the function of islets, so as to provide a basis for studying the internal relationship between immune system and endocrine system. Methods: Isolation of human embryos islets by conventional digestion and primary culture, adding concentration of 10mg / LLPS, the supernatant was harvested at different times, determination of IL 6, insulin, glucagon content and IL 6McAb And experiments, LPS stimulation and control group of islet RNA IL  6RNA spot hybridization. Results: ① The ability of human embryonic islets stimulated IL-6 secretion stimulated by LPS was significantly enhanced in vitro cultured 8d, IL  6 content reached a peak of 145 times that of the control group; â’¡ human embryonic islets in fresh medium with LPS two Sub-stimulation can still continue to secrete high titer of IL 6, the peak time of 25 times the control group; IL 6McAb islet supernatant of IL 6 has a strong neutralizing effect; spot hybridization results showed LPS After stimulated islet RNA IL  6mRNA content was significantly higher than the control group; ⑤ human embryonic islets stimulated by LPS enhanced insulin secretion, glucagon secretion was significantly reduced. Conclusion: Immune mediator LPS may affect pancreatic islet secretion and IL 6 production capacity