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用CTAB法提取野生苎麻总DNA ,结合野生苎麻中酚类、黄酮类等次生代谢物质多的特点 ,加入一定浓度的PVP、β—巯基乙醇 ,可以获得高分子量DNA ,经实验鉴定 ,其纯度符合分子遗传实验要求。
The total DNA of wild ramie was extracted by CTAB method. Combined with more secondary metabolites such as phenols and flavonoids in wild ramie, high molecular weight DNA was obtained by adding PVP and β-mercaptoethanol at a certain concentration. The purity of wild ramie In line with molecular genetic experiment requirements.