论文部分内容阅读
采用聚丙烯酰胺凝胶电泳技术,从西大西洋笛鲷(Lutjanus campechanus)和勒氏笛鲷(L.russel-lii)的微卫星引物中筛选出适用于孟加拉笛鲷(L.bengalensis)和四带笛鲷(L.kasmira)的微卫星引物,并应用于三亚和湛江群体的遗传结构分析.结果表明:25对引物中筛选出13对可以稳定扩增出特异片段的引物,其中12对可在孟加拉笛鲷基因组中扩增出重复性好的特异性条带;10对可在四带笛鲷中扩增出重复性好的特异性条带,并且全部呈现出种内多态;9对为两个种通用.孟加拉笛鲷的湛江群体(ZMJ)和三亚群体(SMJ)、四带笛鲷的湛江群体(ZSD)和三亚群体(SSD)的平均等位基因数分别为:3.888 9、4.111 1、4.333 3、4.222 2;平均观测杂合度分别为:0.583 3、0.638 9、0.594 4、0.638 9;平均多态信息含量分别为:0.472 0、0.469 2、0.547 4、0.525 7.将本实验所得结果与笛鲷鱼其他研究结果进行比较分析,可以看出目前湛江海域野生笛鲷的遗传多样性整体比较丰富,但部分野生笛鲷已表现出杂合子缺失的趋势,这势必会引起将来遗传多样性的降低,所以要尽早对野生群体进行摸底调查,并找出解决养殖群体污染野生群体这一问题的方法.
Using polyacrylamide gel electrophoresis, microsatellite primers from Lutjanus campechanus and L.russel-lii were screened for L. bengalensis and four bands The microsatellite primers of L. kasmira were applied to the genetic structure analysis of Sanya and Zhanjiang populations.The results showed that 13 pairs of primers were screened from 25 pairs of primers for stable amplification of specific fragments, Ten specific bands were amplified from the genome of snapdragon in Bangladesh; 10 pairs of specific bands were amplified with good repeatability in the four sections of snapper, and all of them showed intraspecific polymorphism; 9 pairs were The average number of alleles of ZSD and SMJ in Zengjiang (ZJJIANG) and SZZ (SID) populations of the two common species Bengal snapper were 3.888 9 and 4.111 respectively 1,4.333 and 3,4.222 2 respectively. The average observed heterozygosities were 0.583 3,0.638 9,0.594 4,0.638 9 respectively. The average polymorphic information content was 0.472 0,0.469 2 0.547 4 and 0.525 respectively. The results obtained are compared with other findings of snapper fish and it can be seen that at present Zhanjiang sea area However, some wild snappers have shown the tendency of loss of heterozygotes, which will inevitably lead to the reduction of genetic diversity in the future. Therefore, it is necessary to thoroughly investigate the wild populations and find out the solutions Farming groups pollute the wild population this problem.