目的了解山东地区生猪和家禽屠宰环节沙门菌血清型分布,为进一步开展动物源性产品风险评估提供数据。方法 2014年9~12月从山东省部分屠宰场采集分离的335株沙门菌,应用液态悬浮芯片技术对沙门菌菌体(O)抗原、鞭毛(H)抗原和附加靶蛋白(AT)的基因进行检测,然后运用Salmonella serotyping assay软件分析鉴定血清型。结果 335株沙门菌中299株共分为29个血清型,另有36株未分型,优势血清型主要为德尔卑沙门菌(S.Derby,24.8%,83/335、)和肠炎沙门菌(S.enteritidis,15.2%,51/335)。德尔卑沙门菌、汤卜逊沙门菌(S.Thompson)和阿贡纳沙门菌(S.Agona)在生猪和家禽屠宰场中都有分布;生猪屠宰场以德尔卑沙门菌(39.8%,64/161)为主,其次为鼠伤寒沙门菌(S.typhimurium,19.3%,31/161)和汤卜逊沙门菌(13.7%,22/161);家禽屠宰场中以肠炎沙门菌(28.7%,50/174)、印第安纳沙门菌(S.Indiana,12.1%,21/174)和德尔卑沙门菌(10.9%,19/174)为主。另外,不同地区屠宰场的沙门菌血清型呈差异分布,生猪屠宰场中除鲁中部外,其他地区主要血清型为德尔卑沙门菌;家禽屠宰场中,鲁东和鲁西地区主要是肠炎沙门菌,鲁东北地区以德尔卑沙门菌为主;鲁中部地区分离株以汤卜逊沙门菌为主。结论山东省屠宰场中的沙门菌污染比较严重,且有多种血清型,需加强屠宰环节的致病菌监控,降低动物产品在屠宰环节受沙门菌污染的风险。 Objective To understand the serotype distribution of Salmonella in pigs and poultry slaughtering in Shandong Province and provide data for further risk assessment of animal-derived products. Methods 335 strains of Salmonella isolated from some slaughterhouses in Shandong province from September to December 2014 were used to detect the gene of Salmonella (O) antigen, flagella (H) antigen and additional target protein (AT) using liquid suspension chip technique The assay was followed by analysis of serotypes using the Salmonella serotyping assay software. Results Among the 335 Salmonella isolates, 299 were divided into 29 serotypes and another 36 were not genotyped. The predominant serotypes were Salmonella Delphin (S. Derby, 24.8%, 83/335) and Salmonella Enteritidis (S.enteritidis, 15.2%, 51/335). Salmonella delphi, S. Thompson and S. Agona were found in both live pigs and poultry slaughterhouses; live pig slaughterhouses were distributed in Salmonella delphi (39.8%, 64 / 161), followed by S. typhimurium (19.3%, 31/161) and Salmonella tybus (13.7%, 22/161); Salmonella enteritidis (28.7% , 50/174), S. Indiana (12.1%, 21/174), and Salmonella delphi (10.9%, 19/174). In addition, the slaughterhouse in different regions of Salmonella serogroup showed a difference in distribution, pig slaughterhouse in addition to Lu Central, the main serotypes of other areas of Salmonella delphi; poultry slaughterhouse, Ludong and Luxi region is mainly Enteritis Salmonella Salmonella, Salmonella delphi in northeastern Shandong mainly; Salmonella typhimurium in the central region of Shandong. Conclusion Salmonella in slaughterhouses in Shandong Province is polluted severely and has many serotypes. It is necessary to strengthen the monitoring of pathogens in the slaughtering process and reduce the risk of animal products being contaminated by Salmonella in the slaughtering process.