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目的:观察抗β1肾上腺素受体自身抗体(β1AA)在心律失常患者血清中的分布特征并探究该抗体是否具有致心律失常作用。方法:选择临床各型心律失常、冠心病患者和正常健康体检者,采用酶联免疫吸附法(ELISA)测定血清中β1AA的滴度;提纯抗体阳性患者血清中的IgG抗体,给予正常大鼠,动态监测心电图的变化,观察心律失常发生频率。结果:β1AA在心律失常患者血清中的阳性率为52.8%,明显高于冠心病对照组(24.0%,P<0.01)与正常对照组(5.0%,P<0.01);β1AA可引发正常大鼠发生心律失常,其中以室性心律失常多见。结论:β1AA在心律失常患者血清中的分布呈高阳性率,并可致大鼠发生心律失常。
OBJECTIVE: To observe the distribution of anti-β1 adrenergic receptor autoantibodies (β1AA) in the serum of patients with arrhythmia and explore whether the antibodies have arrhythmogenic effects. Methods: Serum β1AA titers were determined by enzyme-linked immunosorbent assay (ELISA) in patients with various types of arrhythmia, coronary heart disease and normal healthy subjects. The IgG antibodies in the serum of the patients were purified and then were given to normal rats. Dynamic monitoring of ECG changes observed arrhythmia frequency. Results: The positive rate of β1AA in the serum of patients with arrhythmia was 52.8%, which was significantly higher than that of the control group (24.0%, P <0.01) and the normal control group (5.0%, P <0.01) Arrhythmia occurs, of which more common ventricular arrhythmias. Conclusion: The distribution of β1AA in the serum of patients with arrhythmia is highly positive and can cause arrhythmia in rats.