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以药物梯度平皿筛选得到金黄色葡萄球菌NCTC6571、大肠杆菌NCTC10418及普通变形杆菌1065的耐利福定菌株。利福定能明显抑制~3H-尿苷掺入敏感菌株,而对耐药菌株的RNA合成无明显影响;利福定与利福平对标准RNA多聚酶活性的抑制随着药物剂量的增加而加强;提取及纯化RNA多聚酶,利福定能明显抑制敏感菌的酶活性,而对金黄色葡萄球菌NCTC6571与大肠杆菌NCTC10418耐药菌株的酶活性无明显影响。
Screening with drug gradient plates resulted in the production of rifidipine strains of Staphylococcus aureus NCTC6571, E. coli NCTC10418 and Proteus vulgaris 1065. Rifedin significantly inhibited the incorporation of 3H-uridine into susceptible strains, but had no significant effect on the RNA synthesis of drug-resistant strains. The inhibition of standard RNA polymerase activity by rifadin and rifampicin was enhanced with the increase of drug dosage ; Extraction and purification of RNA polymerase, rifamycin can significantly inhibit the activity of sensitive bacteria, and the Staphylococcus aureus NCTC6571 and E. coli NCTC10418 resistant strains of enzyme activity had no significant effect.