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自双相培养基内转种痢疾阿米巴之培养物时,都是自培养基的底部取材,因为底部的虫体较多,但究竟在底部的哪一层,沉淀物曲底呢或表层呢?关于这方面的问题尚缺乏参考资料。本文的研究目的是了解痢疾阿米巴在双相培养基的液体部分中生长和分布的情况,作为转种及浓集虫体的参考。一、方法①培养基是洛克——鸡蛋——血清培养基(1):鸡蛋斜面部分分装于试管(16×1.5厘米)内,每管约1.5毫升,斜面高度约4厘米;复盖液为5毫升(洛克氏液∶牛血清=8∶1);米粉1白金耳。②痢疾阿米巴培养48小时后,在培养基试管外壁,用蜡笔划分6个不同的水位标记(图1),即:Ⅰ.沉淀物下层;Ⅱ.沉淀物上层;Ⅲ.沉淀物表层——紧接触沉淀物上层之一薄水膜;Ⅳ.沉淀物上部1—5毫米处;
Transgenic cultures of dysentery amoeba from a biphasic medium are drawn from the bottom of the medium because there are more parasites at the bottom, but which layer at the bottom, What? There is still no reference material on this issue. The aim of this study was to understand the growth and distribution of amoebae in the liquid part of a biphasic medium as a reference for the transformation and concentration of parasites. First, the method ① medium is Locke - egg - serum medium (1): the bevel part of the egg is divided into test tubes (16 × 1.5 cm), each tube is about 1.5 ml, the bevel height of about 4 cm; 5 ml (Locke’s solution: bovine serum = 8: 1); rice flour 1 platinum ear. ② After 48 hours of culture of Amoeba diarrhea, six different water level markers (Fig. 1) were divided by crayon on the outer wall of the culture tube, namely: Ⅰ. Precipitate lower layer Ⅱ Upper sediment layer Ⅲ Precipitate surface - - a thin film of water immediately above the precipitate; IV. 1-5 mm above the precipitate;