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目的:研究ABO及RhD血型快速鉴定纸卡的稳定性,以改进和确定试剂的存放条件和有效期。方法:选择3批血型快速鉴定纸卡,分别存放于-40℃、2~8℃、20~25℃、37℃、60℃等不同温度条件下,于放置的不同时间分别进行外观、特异性与亲和力检测。结果:ABO、RhD血型快速鉴定纸卡分别在-40℃、2~8℃、20~25℃、37℃、60℃条件下放置1个月、2个月后,外观与1周内制备的纸卡基本一致;特异性均合格,1min凝集强度均合格。结论:该ABO、RhD血型快速鉴定纸卡制备技术稳定,在-40~60℃存放2个月,对纸卡中的试剂无显著影响。
OBJECTIVE: To study the stability of ABO and RhD blood type rapid identification paper cards to improve and determine the reagent storage conditions and expiration date. Methods: Three batches of blood type quick identification paper cards were selected and stored at different temperatures of -40 ℃, 2 ~ 8 ℃, 20 ~ 25 ℃, 37 ℃, 60 ℃, respectively. The appearance, specificity With affinity test. Results: The ABO and RhD blood type quick identification paper cards were respectively placed in the condition of -40 ℃, 2 ~ 8 ℃, 20 ~ 25 ℃, 37 ℃, 60 ℃ for 1 month. After 2 months, Paper card basically the same; specificities are qualified, 1min agglutination strength are qualified. Conclusion: The ABO and RhD blood type rapid identification paper card preparation technology is stable, stored at -40 ~ 60 ℃ for 2 months, no significant effect on the reagents in the paper card.