目的:诱导杜仲毛状根的发生及筛选优良毛状根系。方法:利用发根农杆菌C58C1诱导杜仲不同外植体产生毛状根,并利用HPLC测定桃叶珊瑚苷含量。结果:采用杜仲无菌苗胚轴经菌液浸染20min,共培养3d可得到最佳转化效果。液体培养的生长量优于固体培养,最有利于毛状根生物量和药用成分的积累,并筛选到桃叶珊瑚苷高产根系E1。结论:可通过毛状根的培养来获得桃叶珊瑚苷药用成分。 Objective: To induce hairy roots of Eucommia and to screen excellent hairy root system. Methods: Hairy roots of different explants of Eucommia ulmoides were induced by Agrobacterium rhizogenes C58C1 and the content of aucubin was determined by HPLC. Results: The best transfection effect was obtained by using eucalyptus sterile seedling embryo axis for 20min by bacterial culture. The growth of liquid culture is better than solid culture, which is most conducive to the accumulation of hairy root biomass and medicinal components, and screened aucubin high yielding root system E1. Conclusion: The aucubin medicinal ingredients can be obtained through the culture of hairy roots.