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以采集于2007年和2010年的油松成熟种胚为外植体,研究了不同激素组合及浓度对外植体愈伤组织分化、不定芽诱导和生根的影响。研究结果表明:诱导愈伤组织的适宜培养基为1/2MS+6-BA1.5mg.L-1+NAA0.2mg.L-1+蔗糖30g.L-1+琼脂6g.L-1,2007年和2010年的成熟种胚愈伤诱导率分别为33.33%和54.17%。诱导不定芽最佳光培养条件是12/12h,适宜的培养基为1/2MS+6-BA1.5mg.L-1+NAA0.1mg.L-1+蔗糖30g.L-1+琼脂6g.L-1,2007年和2010年的种胚愈伤诱导的不定芽分化率分别为50.0%和58.3%。生根培养效果较好的培养基是DCR+IBA0.1mg.L-1+NAA0.1mg.L-1+KT0.1mg.L-1+蔗糖30g.L-1+琼脂6g.L-1,2007年和2010年的种胚愈伤诱导的不定芽生根率分别为36.4%和18.2%。
Taking the mature embryos of Pinus tabulaeformis collected in 2007 and 2010 as explants, the effects of different hormone combinations and concentrations on callus differentiation, induction of adventitious buds and rooting of explants were studied. The results showed that the suitable culture medium for inducing callus was 1 / 2MS + 6-BA1.5mg.L-1 + NAA0.2mg.L-1 + sucrose30g.L-1 + agar6g.L-1,2007 The callus induction rate of mature embryos in 2010 and 2010 were 33.33% and 54.17% respectively. The optimum light culture conditions for adventitious buds induction were 12 / 12h, and the suitable culture medium was 1 / 2MS + 6-BA1.5mg.L-1 + NAA0.1mg.L-1 + sucrose30g.L-1 +6g agar. The embryogenic callus-induced adventitious bud differentiation rates in L-1,2007 and 2010 were 50.0% and 58.3%, respectively. The medium with better rooting culture was DCR + IBA0.1mg.L-1 + NAA0.1mg.L-1 + KT0.1mg.L-1 + sucrose30g.L-1 + agar6g.L-1,2007 The callus induction rate of embryogenic calli in year and 2010 were 36.4% and 18.2% respectively.