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目的探讨膳食补充营养素可能对持续高 +Gz暴露后小鼠脑功能的影响 ,用迷宫实验评价脑功能状况。方法将小鼠分为对照组 (A组 ) ,+Gz暴露但不补充营养素组 (B组 )及 +Gz暴露并且补充营养素组(C组 )。每组 1 2只小鼠。A组不作 +Gz暴露 ,B组和C组 +1 0Gz暴露 8min。B组在 +Gz暴露前 3h以 0 .3ml蒸馏水灌胃。C组在 +Gz暴露前 1d饮用强化VitB6 的水 ,+Gz暴露前 3h以 0 .3ml混合氨基酸液灌胃。记录 3组动物的迷宫实验成绩。迷宫实验后立即摘眼球取血 ,测定氨基酸 ;断头取脑组织 ,制成组织匀浆 ,测定单胺类递质。结果 +Gz暴露后 ,与对照组相比B组迷宫实验的完成时间显著延长 ,错误次数增加 ,C组迷宫实验成绩有改善的趋势 ,即完成时间缩短 ,错误次数减少。与B组相比 ,C组小鼠脑组织中 5 羟色胺 (5 HT)与多巴胺 (DA)的比值显著下降。B组和C组脑内γ谷氨酰基转移酶 (GGT)活性显著增加。结论持续高 +Gz暴露显著降低小鼠迷宫实验成绩。膳食补充某些氨基酸和VitB6 有改善迷宫成绩的趋势 ,可能与小鼠脑组织中 5 HT与DA的比值显著下降有关
Objective To investigate the effect of dietary supplementation on brain function in mice exposed to sustained high + Gz exposure and to evaluate brain function by maze test. Methods The mice were divided into control group (group A), + Gz exposed but not supplemented group (group B) and + Gz exposed and supplemented group (group C). Twelve mice per group. Group A did not give + Gz exposure, Group B and C +10 Gz exposure for 8 minutes. Group B before + Gz exposure 3h 0 .3ml distilled water gavage. Group C was given VitG6 water one day before exposure to + Gz, and 0 .3 ml mixed amino acid solution was given 3 hours before Gz exposure. The maze test scores of three groups of animals were recorded. Maze test immediately after taking the eye blood, determination of amino acids; decapitated brain tissue, made of tissue homogenates, determination of monoamine neurotransmitters. Results After + Gz exposure, the completion time of maze test in Group B was significantly longer and the number of errors increased compared with the control group. The maze test scores in Group C tended to improve, ie, the completion time was shortened and the number of errors was decreased. Compared with group B, the ratio of serotonin (5 HT) to dopamine (DA) in group C mice significantly decreased. B group and C group brain gamma glutamyl transferase (GGT) activity was significantly increased. Conclusions Sustained high + Gz exposure significantly reduced maze test scores in mice. Dietary supplementation of certain amino acids and VitB6 have a tendency to improve maze scores and may be related to a significant decrease in the ratio of 5 HT to DA in mouse brain tissue