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以 BC_3HI 肌细胞为模型,用膜片-电压固定(patch-clamp)技术,研究观察在单一细胞或膜单通道的Ca~(2+)、Na~+和 K~+的变化。肌肉的分化与膜的电压-门控离子通道(voltage-gated ion channels)的表达是依赖于促细胞分裂剂的消退和细胞的生长停滞。通常,电压-门控通道能诱发肌细胞的特异基因产物,但其致癌性的基本机理仍不明。分化的 BC_3HI 肌细胞表达了机能性的 Ca~(2+)和 Na~+通道,当细胞生长增殖和为某些等位癌基因转染时,机能性的离子通道被阻抑。这种机能性的 Ca~(2+)和 Na~+通道,在促细胞分裂剂消退约5天后,才首次检出Ca~(2+)和 Na~+的内向电流。在促细胞分裂剂消退时,暂时诱发 BC_3HI 细胞的电压-门控通道。为了试验细胞癌基因是否能阻止膜离子通道的表达,以 BC_3HI 细胞的克隆细胞株,即以 BC_3HI 细胞,用癌基因表达载
The changes of Ca ~ (2 +), Na ~ + and K ~ + in a single cell or membrane were observed by BC-3HI muscle cell patch-clamp technique. Muscle differentiation and the expression of voltage-gated ion channels in membranes are dependent on the regression of mitogen and the arrest of cell growth. In general, voltage-gated channels induce specific gene products of muscle cells, but the underlying mechanism of carcinogenicity remains unclear. The differentiated BC3HI muscle cells express functional Ca2 + and Na ~ + channels, and functional ion channels are repressed as cell growth and proliferation are transplanted for certain allelic oncogenes. The functional Ca 2+ and Na + channels first detected the inward currents of Ca 2+ and Na + within about 5 days after the mitogen had dissipated. Upon mitigation of mitogen, the voltage-gated channels of BC_3HI cells were transiently induced. To test whether the oncogene can block the expression of membrane ion channels, the cloned cell line BC_3HI cells, ie, BC_3HI cells,