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目的 合成并鉴定已预测出的 4个MAGE 3HLA A2限制性CTL表位多肽 ,为后续研究奠定基础。方法 采用固相合成法合成多肽 ,经RP HPLC纯化、纯度分析 ,用质谱进行定性鉴定。结果 4个合成肽的纯度都在 95 %以上 ,经质谱分析 ,各肽的分子量测定值与理论值相符。结论 所得多肽为高纯度的目标肽 ,为后续研究提供了可靠的保证
Objective To synthesize and identify four predicted MAGE 3HLA A2 restricted CTL epitope peptides, which will lay a foundation for further research. Methods Polypeptides were synthesized by solid-phase synthesis, purified by RP HPLC, purity analysis and qualitative identification by mass spectrometry. Results The purity of the four synthesized peptides was above 95%. Mass spectrometry analysis showed that the measured molecular weights of the peptides were in accordance with the theoretical values. Conclusion The resulting peptide is a high purity target peptide, which provides a reliable guarantee for subsequent studies.