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目的比较不同代次的狗肾传代(MDCK)细胞对流感病毒的敏感性差异。方法选择生长状态及形态良好的、不同代次的MDCK细胞,在相同培养条件下同时接种流感样病例咽拭子标本,观察细胞病变,细胞收获后采用血凝试验测定病毒血凝效价和分离率;以参考流感病毒株测定TCID50值,比较不同代次MDCK细胞对流感病毒的敏感性。结果 3个不同代次的MDCK细胞对流感样病例标本的分离阳性率、血凝效价相比差异无统计学意义,且3者TCID50值相比,相差不超出1个Lg。结论在相同实验条件以及细胞生长状态和形态良好的情况下,不同代次的MDCK细胞对流感病毒的敏感性无差异。
Objective To compare the sensitivity of MDCK cells to influenza virus in different generations. Methods The MDCK cells with different morphologies and morphologies were selected and inoculated with throat swab specimens of influenza-like cases under the same culture conditions to observe the cytopathic effect. After the cells were harvested, the hemagglutination titer and separation of the virus were determined by hemagglutination test Rate; TCID50 value was measured with reference to the influenza virus strain and the susceptibility to influenza virus in different generations of MDCK cells was compared. Results There was no significant difference in the positive rates of the separation and the hemagglutination titer between three different generations of MDCK cells and the TCID50 values of the three patients did not differ more than one Lg. Conclusion The MDCK cells of different generations showed no difference in sensitivity to influenza virus under the same experimental conditions and cell growth status and morphology.