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目的:考察保存条件和保存时间对环孢素全血标本浓度的测定结果的影响。方法:采用荧光偏振免疫法(FPIA)测定肾移植术后患者的全血标本浓度,然后将标本分别置于室温或冰箱冷藏2周,再次测定标本浓度,考察其稳定性。结果:室温下1周和冷藏2周的标本测定结果与保存前均无统计学差异(P>0．05),且冷藏者变异较小。结论:全血标本在室温下可稳定保存1周,在冷藏下至少可稳定保存2周,冷藏仍是较好保存方法。
OBJECTIVE: To investigate the effect of storage conditions and storage time on the determination of the concentration of CsA in whole blood samples. Methods: FPA was used to determine the concentration of whole blood in patients after renal transplantation. The specimens were placed in room temperature or refrigerator for 2 weeks respectively, and then the concentration of the samples was measured again to investigate the stability. Results: There was no significant difference (P> 0.05) between the results of samples stored at room temperature for 1 week and those stored for 2 weeks, and the variation of frozen samples was less. Conclusion: The whole blood samples can be stored stably for 1 week at room temperature, and can be stored stably for at least 2 weeks under refrigeration.