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提出用表征酶催化反应速度的参数()与表征扩散速度的参数(’)之比作为酶电极特性参数(φ)来表述酶电极的基本方程具有优点,导出了借助此参数表示酶电极电位响应值与响应时间的方程式,讨论了影响因素。对于电流测量法酶电极的二种基本情况也均可用参数(?)及(?)以相似的形式表述电流响应值,其比值为一简单数值φ/2。证明在电位测量法中当响应时间大于1.7τ(=(?))时可认为已达稳态(仅差1毫伏,φ>1.7),但电位值较最大值低17.5毫伏。证明φ值虽变化达三个数量级,响应时间变化仍不到一倍。
It is an advantage to present the basic equation of the enzyme electrode with the parameter () characterizing the enzyme-catalyzed reaction and the parameter ( ’) characterizing the diffusion rate as the enzyme electrode parameter (φ) Potential response and response time of the equation to discuss the impact of factors. For the two basic cases of the current-measuring enzyme electrode, the current response can be expressed in a similar way with the parameters (?) And (?) At a simple value of φ / 2. It turns out that the steady-state (1 mV, 1 mV, 1.7> mV) is considered as the response time greater than 1.7τ (= (?)) In potentiometry, but the potential is 17.5 millivolts below the maximum. It is proved that although the value of φ changes by three orders of magnitude, the change of response time is still less than double.