论文部分内容阅读
目的:观察双氢青蒿素对雄激素非依赖性前列腺癌细胞株PC-3M细胞凋亡和血管内皮生长因子(VEGF)表达的影响。方法:不同浓度(0、25、50、100μmol/L)的双氢青蒿素分别作用于PC-3M细胞48 h,MTT法检测细胞生长活性;流式细胞仪测定细胞凋亡率;分光光度法检测细胞凋亡过程中caspase-3、caspase-8活性变化;半定量RT-PCR法检测PC-3M细胞内VEGF mRNA的表达;Western印迹法检测细胞VEGF蛋白表达。结果:双氢青蒿素能显著抑制PC-3M细胞的增殖,与对照组(0μmol/L)的细胞凋亡率(2.92±0.45)%相比,各剂量组(25、50、100μmol/L)的细胞凋亡率[(8.85±0.74)%,(12.83±0.84)%,(18.65±1.24)%]显著增加,caspase-8[(0.47±0.05)U/μg vs(1.22±0.15)U/μg,(1.76±0.07)U/μg,(2.91±0.24)U/μg]、caspase-3[(0.44±0.07)U/μg vs(0.95±0.08)U/μg,(1.48±0.14)U/μg,(2.92±0.45)U/μg]活性显著增加,呈剂量依赖性(P<0.01)。PC-3M细胞内VEGF mRNA的表达和蛋白表达呈剂量依赖性降低。结论:双氢青蒿素能显著抑制体外PC-3M细胞的生长,并促进其凋亡,机制可能与增加凋亡蛋白酶和抑制VEGF表达有关。
Objective: To observe the effect of dihydroartemisinin on the apoptosis and the expression of vascular endothelial growth factor (VEGF) in androgen-independent prostate cancer cell line PC-3M. METHODS: PC-3M cells were treated with dihydroartemisinin at different concentrations (0, 25, 50 and 100μmol / L) for 48 h, respectively. Cell viability was measured by MTT assay. Apoptosis rate was measured by flow cytometry. The changes of caspase-3 and caspase-8 activities in apoptosis were detected by semi-quantitative RT-PCR. The expression of VEGF mRNA in PC-3M cells was detected by Western blotting. RESULTS: Dihydroartemisinin could significantly inhibit the proliferation of PC-3M cells. Compared with the control group (2.92 ± 0.45)%, the apoptosis rate of PC-3M cells was significantly increased (25,50,100μmol / L ) Were significantly higher than those in control group ([(8.85 ± 0.74)%, (12.83 ± 0.84)%, (18.65 ± 1.24)%] (1.66 ± 0.07) U / μg, (2.91 ± 0.24) U / μg], caspase-3 [(0.44 ± 0.07) (292 ± 0.45) U / μg] in a dose-dependent manner (P <0.01). The expression of VEGF mRNA and the protein expression of PC-3M cells decreased in a dose-dependent manner. CONCLUSION: Dihydroartemisinin can significantly inhibit the growth of PC-3M cells and promote its apoptosis in vitro. The mechanism may be related to the increase of protease and the inhibition of VEGF expression.