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①目的了解人胚胎期角膜神经的发育和分布方式，为临床角膜疾病、角膜损伤后的神经再生和感觉恢复的研究提供依据。②方法用氯化金神经染色法和连续冰冻切片技术，在光学显微镜下观察１６周和２６周人胎儿角膜神经的发育和分布。③结果１６周和２６周胎儿角膜中均见有一连续的角膜周缘神经丛。自此神经丛发出的神经纤维分３条途径进入角膜上皮，即经结合膜直接进入角膜上皮周缘区；经巩膜和基质浅层进入角膜上皮并分布至上皮周围的２／３区，经基质分布至角膜上皮的中央区。角膜内皮层未见有神经分布。④结论１６周和２６周胎儿角膜神经首先出现于上皮并形成神经网，然后在基质出现少量神经干，内皮没有神经分布。从上皮层至内皮层神经纤维的数量依次递减。
Objective To understand the development and distribution of corneal nerves in human embryonic corneas, and to provide basis for clinical corneal diseases, nerve regeneration and sensory recovery after corneal injury. Methods The development and distribution of human fetal corneal nerves at 16 weeks and 26 weeks were observed under a light microscope with gold chloride staining and continuous frozen sections. Results 16 weeks and 26 weeks fetal cornea are seen in a continuous corneal peripheral plexus. Since then the nerve fibers issued by the nerve fibers into the corneal epithelium in three ways, that is, directly into the corneal epithelial border zone through the junctional membrane; the scleral and stromal shallow into the corneal epithelium and distribution to the epithelial 2/3 area around the matrix distribution To the central area of the corneal epithelium. There was no nerve distribution in corneal endothelium. Conclusions Fetal corneal nerves first appeared in the epithelium and formed neural networks at 16 weeks and 26 weeks. Then, a small amount of neural stem cells appeared in the stroma, and there was no nerve distribution in the endothelium. The number of nerve fibers from the epithelium to the endodermis successively decreases.