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采用Raman光谱从分子水平揭示了竹红菌乙素光敏损伤的人红细胞膜发生膜蛋白交联和膜脂脂质过氧化导致其功能变化 ;膜流动性和离子通透性增加的本质是竹红菌乙素产生的活性氧 ( 1O2 ,O2 ·和·OH等 )破坏了红细胞膜的有序结构 ,使膜蛋白主链结构的α 螺旋、β 折叠明显减少 ,无规卷曲增加并使其侧链结构的巯基基团、吲哚环、对羟苯基环、单基取代苯基环等也明显减少 .与此同时 ,随着光照时间的增加 ,膜脂的反式构象呈先增加后减少的趋势 ,它的扭曲构象则正好相反 .膜蛋白和膜脂构象不灵敏的CH2 和CH3弯曲振动谱线的明显下降 ,揭示它们有链的断裂 .
Raman spectroscopy revealed at molecular level Hypocrellin B photosensitized human erythrocyte membrane protein crosslinking and membrane lipid peroxidation led to its functional changes; membrane fluidity and the essence of increased ion permeability is bamboo red The reactive oxygen species (1O2, O2 · and · OH, etc.) produced by the bacitracin destroy the ordered structure of the erythrocyte membrane, significantly reducing the α-helix and β-sheet of the membrane protein backbone structure, increasing the random coil, The structure of the mercapto groups, indole ring, p-hydroxyphenyl ring, monosubstituted phenyl ring also significantly reduced at the same time, with the increase of light time, the trans conformation of the membrane lipid increased first and then decreased Trend, its twisted conformation is exactly the opposite.Mechanical membrane protein and membrane lipid conformation of the CH2 and CH3 bending vibration line significantly decreased, revealing their chain fracture.