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赤霉素(GA)促进葡萄果实的膨大,目前对外源赤霉素促进葡萄果实膨大的信号转导途径还不完全清楚。有研究发现GA3处理后葡萄果实中一个SCARECROW(SCR)蛋白的表达出现上调,为了进一步揭示GA的作用途径,本研究通过分析在其他植物中已经确认的SCR基因序列,对葡萄全基因组进行BLAST比对,获得了可能编码葡萄SCR的11个基因片段序列SCRs。分别设计特异性引物,以无核白鸡心葡萄(Vitis vinifera L.cv.Centennial seedless)不同组织器官,以及盛花后12d以30mg/L GA3处理无核白鸡心葡萄的花序,并于花后13、15、19、45、61d采收的果实为材料,进行半定量RT-PCR测定SCRs的表达。结果显示:这些基因在成叶、幼叶、根、花序、新梢顶尖、休眠芽、胚性愈伤组织和非胚性愈伤组织中存在差异表达;在花后13、19、45、61d的葡萄果实中有不同状态的上调。研究结果对进一步探讨SCRs在葡萄果实发育和成熟过程的作用与功能提供了线索。
Gibberellin (GA) promotes the enlargement of grape fruit. At present, the signal transduction pathway of exogenous GA3 to promote grape fruit enlargement is not fully understood. It was found that the expression of SCARECROW (SCR) protein was up-regulated in grape fruits treated with GA3. To further reveal the pathway of GA, the present study analyzed the whole genome of grape by BLAST ratio Yes, 11 gene fragment sequences SCRs that may encode grape SCRs were obtained. The specific primers were designed respectively to treat inflorescences of different tissues and organs of Vitis vinifera L.cv.Centennial seedless, The fruits harvested at 15, 19, 45, and 61d were used as materials to detect the expression of SCRs by semi-quantitative RT-PCR. The results showed that these genes were differentially expressed in the leaves, young leaves, roots, inflorescences, shoot tips, dormant buds, embryogenic callus and non-embryogenic callus. At 13, 19, 45, The grapes have different states of fruit up. The results provide clues to further explore the function and function of SCRs in grape fruit development and maturation.