采用阿斯贝培养基进行菌种分离纯化,再通过吲哚试验、糖发酵试验等9个试验对其进行生理生化鉴定,最终得到一株革兰氏阴性,无芽孢的短杆状固氮菌。试验为微生物固氮菌肥生产中菌种的筛选提供参考,为商业化生产优质的生物固氮菌肥提供基础。 The Aspergillus culture medium was used to separate and purify the strain, and then physiological and biochemical identification was carried out by nine experiments including indole test and sugar fermentation test. Finally, a gram-negative and spore-free short rod-shaped azotobacter was obtained. The experiment provides a reference for the selection of strains for microbial nitrogen-fixing fertilizer production, and provides the basis for the commercial production of high quality nitrogen-fixing bacterial fertilizer.