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目的观察共刺激分子4-1BBL基因导入小鼠胃癌MFC细胞后在小鼠体内诱导的抗肿瘤效应以及对小鼠免疫状况的影响。方法用脂质体法把pMKITneo/4-1BBL和pMKITneo质粒导入615小鼠胃癌细胞MFC内,再用MFC、MFC/pMKITneo和MFC/4-1BBL细胞分别接种615小鼠,观察其成瘤情况,并测定肿瘤细胞的凋亡率及外周血的CD4~+、CD8~+T细胞和NK细胞的含量。结果4-1BBL基因转染后的胃癌MFC细胞在615小鼠体内成瘤所需的时间长、瘤体的重量轻；且MFC/4-IBBL组的癌细胞凋亡率(22．45±4．11)%明显高于MFC组(18．47±3．34)%及MFC/pMKITneo组(17．87±4．04)%(P＜0．05)；MFC、MFC/pMKITneo组小鼠外周血CD8~+T、NK细胞数明显低于正常对照组和MFC/4-1BBL组(P＜0．05),且MFC、MFC/pMKITneo两组间差异无统计学意义(P＞0．05)。结论4-1BBL基因导入胃癌MFC细胞后能够提高荷瘤机体的免疫能力、延缓肿瘤的发生、抑制肿瘤的发展、促进肿瘤细胞的凋亡。
Objective To observe the antitumor effect induced by co-stimulatory molecule 4-1BBL gene in mice gastric cancer MFC cells and its effect on immune status in mice. Methods The pMKITneo / 4-1BBL and pMKITneo plasmids were introduced into the MFC of 615 mice gastric cancer cells by lipofectamine. The 615 mice were inoculated with MFC, MFC / pMKITneo and MFC / 4-1BBL cells respectively to observe their tumorigenicity, The apoptosis rate of tumor cells and the content of CD4 ~ +, CD8 ~ + T cells and NK cells in peripheral blood were measured. Results The gastric cancer MFC cells transfected with 4-1BBL gene took a long time to tumorigenicity in 615 mice and the tumor weight was light. The apoptosis rate of MFC cells in MFC / 4-IBBL group (22.45 ± 4 .11%) was significantly higher than that in MFC group (18.47 ± 3.34)% and MFC / pMKITneo group (17.87 ± 4.04)% (P <0.05) The numbers of CD8 ~ + T and NK cells in peripheral blood were significantly lower than those in normal control group and MFC / 4-1BBL group (P <0.05). There was no significant difference between MFC and MFC / pMKITneo groups (P> 0.05). 05). Conclusion The introduction of 4-1BBL gene into gastric cancer cells can improve the immunity of tumor-bearing cells, delay the occurrence of tumors, inhibit the development of tumors and promote the apoptosis of tumor cells.