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目的探讨核因子κB在羊水入血后的大鼠肺组织中的活性变化及地塞米松的干预作用。方法Wistar雌性大鼠30只制成大鼠羊水入血模型,其中16只为羊水组、14只按01mg/100g注入地塞米松注射液作为羊水+地塞米松组;Wistar雌性大鼠34只制成大鼠胎粪入血模型,其中20只为胎粪组、14只按01mg/100g注入地塞米松注射液作为胎粪+地塞米松组。另选6只Wistar雌性大鼠作为对照组。模型制成60min时,采用注射10%氯化钾的方法处死大鼠,提取大鼠肺组织行病理检查。采用免疫印迹法检测大鼠肺组织中的核因子κB结合活性;免疫组化方法检测大鼠肺组织核因子κB阳性细胞率。结果(1)羊水组及胎粪组大鼠肺组织有不同程度水肿,血管丰富,局部可见少量出血,血管及支气管周围可见大量炎性细胞浸润(主要为中性粒细胞);羊水+地塞米松组及胎粪+地塞米松组肺组织病理改变均较羊水组及胎粪组减轻;对照组大鼠肺组织则无明显改变。(2)胎粪组肺组织中核因子κB结合活性为438698±13092,明显高于羊水组的377982±7445及对照组的267691±12382,两者比较,差异有统计学意义(P<001);羊水+地塞米松组及胎粪+地塞米松组肺组织中核因子κB结合活性(分别为308826±13771及339516±17358)均较羊水组及胎粪组显著降低,两者比较,差异有统计学意义(P<005)。(3)?
Objective To investigate the changes of the activity of NF-κB in rat lung after amniotic fluid infiltration and the effect of dexamethasone. Methods 30 Wistar female rats were made into amniotic fluid into the blood model, of which 16 were amniotic fluid groups, 14 were injected with dexamethasone injection of 01mg / 100g as amniotic fluid + dexamethasone group; 34 Wistar female rats Into the rat meconium into the blood model, of which 20 were meconium-only group, 14 were injected 01mg / 100g dexamethasone injection as meconium + dexamethasone group. Six other Wistar female rats were selected as control group. When the model was made 60min, the rats were sacrificed by injection of 10% potassium chloride, and the lung tissues of rats were taken for pathological examination. The NF-κB binding activity in rat lung was detected by Western blotting. The expression of NF-κB in rat lung was detected by immunohistochemistry. Results (1) The lung tissue of amniotic fluid and meconium group had different degrees of edema, abundant blood vessels and a small amount of local bleeding, and a large amount of inflammatory cell infiltration (mainly neutrophils) around blood vessels and bronchus; amniotic fluid + The pathological changes of lung in the group of Meisong and meconium + dexamethasone were less than those of the amniotic fluid and group of meconium. The lung tissue of the control group had no significant change. (2) The NF - κB binding activity in the lung tissue was 438698 ± 13092, significantly higher than 377982 ± 7445 in the amniotic fluid group and 267691 ± 12382 in the control group. The difference was statistically significant (P <0.001). The NF-κB binding activity (308826 ± 13771 and 339516 ± 17358, respectively) in amniotic fluid + dexamethasone group and meconium + dexamethasone group was significantly lower than that in amniotic fluid group and meconium group, with statistical difference Significance of learning (P <005). (3)?