七十年代建立起来的体外人精子穿透无透明带仓鼠卵试验(简称SPA),是评定精子授精能力的新技术。此法较完整地体现了包括精子获能、顶体反应、精卵膜融合和协调的鞭毛运动等精子功能的生物学现象。已证实,与常规精液分析不同,SPA试验与精子授精能力有良好的相关关系。特别适用于评定反常的精子功能。本室SPA操作程序与国内外同类实验不尽相同,故介绍如下。 Seventies set up in vitro human sperm penetration no-band hamster egg test (SPA), is to assess sperm fertilization ability of the new technology. This method more fully embodies biological phenomena including sperm capacitation, acrosome reaction, ectomembrane fusion and coordinated flagella motility and other sperm functions. It has been shown that, unlike conventional semen analysis, the SPA test has a good correlation with sperm fertility. Particularly suitable for assessing abnormal sperm function. This room SPA procedures and experiments at home and abroad are not the same, it is described below.