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目的:建立荆防小儿止咳口服液中总皂苷含量测定的方法。方法:采用AB-8型大孔吸附树脂分离和纯化口服液中三萜类总皂苷,以桔梗皂苷D为对照品,香草醛-高氯酸为显色剂,采用比色法于476nm处测定口服液中总皂苷的含量。结果:标准曲线在0.0516~0.3096mg范围内线性关系良好,平均加样回收率为99.20%,RSD为1.48%,且该方法在60min内稳定。结论:该方法灵敏、简便、快速、重复性好,可作为荆防小儿止咳口服液中总皂苷的含量测定方法。
Objective: To establish a method for determination of total saponin in Jing-Fang Xiaoer Zhike Oral Liquid. Methods: The AB-8 macroporous resin was used to separate and purify triterpene saponins in oral solution. Platycodin D was used as reference substance and vanillin-perchloric acid was used as color reagent. The colorimetric method was used to determine the content of triterpenoids at 476 nm Oral liquid total saponin content. Results: The calibration curve showed a good linearity in the range of 0.0516-0.3096 mg with an average recovery of 99.20% and a RSD of 1.48%. The method was stable within 60 min. Conclusion: The method is sensitive, simple, rapid, reproducible, and can be used as a method for the determination of total saponins in Jing anti-pediatric cough oral liquid.