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目的探讨兔肝VX2瘤MR灌注成像(PWI)动态变化的病理机制,以及PWI在评价肝脏肿瘤血管生成中的应用价值。方法建立15只兔肝VX2瘤模型,随机分成A、B、C3组,每组5只,分别在肿瘤种植成功后16、28d和45d对肿瘤进行PWI,随后处死荷瘤兔,并对瘤标本行免疫组化微血管密度(MVD)检测及H-E染色。PWI采用信号强度-时间曲线的最大信号下降斜率(SRSmax)作为定量指标,分析肿瘤SRSmax与MVD的相关性,并分析肿瘤PWI表现与病理特征的关系。结果除C组肿瘤周边区与瘤旁肝实质MVD计数的差异无统计学意义外(P>0.05),余各组内VX2瘤中央区、周边区及瘤旁肝实质的SRSmax、MVD的差异有统计学意义(P<0.05)。肿瘤的SRSmax与MVD呈正相关(r=0.731,P=0.002)。3组之间肿瘤的SRSmax、MVD的差异有统计学意义(P<0.05),A组的SRSmax、MVD明显高于C组(P<0.05)。结论兔肝VX2瘤PWI表现与其病理特征相符,PWI可用于评价肝脏肿瘤的血管生成状况。
Objective To investigate the pathological mechanism of dynamic MR perfusion imaging (PWI) in rabbit liver VX2 tumor and the value of PWI in assessing hepatic tumor angiogenesis. Methods Fifteen rabbit models of VX2 liver tumor were established and randomly divided into groups A, B and C3, with 5 rats in each group. PWI was performed on the 16th, 28th and 45th day after tumor implantation, and then the tumor-bearing rabbits were sacrificed. Immunohistochemical staining of microvessel density (MVD) and HE staining were performed. PWI used the maximum signal descent slope (SRSmax) of signal intensity vs. time curve as a quantitative index to analyze the correlation between tumor SRSmax and MVD, and analyzed the relationship between tumor PWI and pathological features. Results The differences of SRSmax and MVD in the central area, peripheral area and peritumoral parenchyma of the VX2 tumor in the remaining groups were not statistically significant except for the MVD count in the peripheral area of the tumor and the para-tumorous liver in the C group (P> 0.05) Statistical significance (P <0.05). There was a positive correlation between SRSmax and MVD (r = 0.731, P = 0.002). There were significant differences in SRSmax and MVD between the three groups (P <0.05). The SRSmax and MVD in group A were significantly higher than those in group C (P <0.05). Conclusion The PWI of rabbit VX2 tumor is consistent with its pathological features. PWI can be used to evaluate the angiogenesis of liver tumors.