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用改造后的静脉留置针将地鼠气管腹侧面约1/4的粘膜刮除,取材前1h腹腔内注入Brdu气管标本经HE、PAS,抗Brdu免疫染色,光、电镜观察。损伤后6h开始,边缘部上皮细胞增殖并向损伤面内爬行,胞浆呈PAS阳性,核呈Brdu阳性,24h覆盖损伤面。电镜下这些细胞表面无纤毛或仅有少量微绒毛,浆内有大小不等散在的粘液分泌颗粒和较多的粗面内质网。36h开始,损伤面由3一5层表皮样化生细胞构成,核周出现张力丝状物围绕,部分胞浆内仍有少量粘液颗粒。从72h开始,细胞出现短而细的纤毛或出现较多的粘液分泌空泡。2w后恢复正常气管上皮结构。这表明气管上皮中的粘液分泌细胞不是终末细胞,具有很强的DNA合成和增殖能力,是损伤后参与修复的主要细胞之一。
The modified venous catheter was used to remove about 1/4 of the mucous membrane from the ventral surface of the hamster trachea. Brdu tracheal samples were intraperitoneally injected with HE, PAS, anti-Brdu immunostaining, light and electron microscopy 1 h before drawing. At 6h after injury, the epithelial cells in the marginal part proliferated and crawled in the injured area. The cytoplasm showed positive PAS, Brdu positive in the nucleus and covered the damaged area in 24h. Electron microscopy of these cells on the surface without cilia or only a small amount of microvilli, the size of the slurry within the scattered size of the mucus secreted particles and more rough endoplasmic reticulum. 36h, the injury surface consists of 3 to 5 epidermis-like metaplastic cells, around the nucleus there is tension filaments around, some of the cytoplasm is still a small amount of mucus particles. Starting from 72h, the cells appeared short and thin cilia or more mucus secreted vacuoles. After 2w to restore normal tracheal epithelial structure. This indicates that the mucous secreting cells in the tracheal epithelium are not terminal cells and have strong ability of DNA synthesis and proliferation. They are one of the main cells involved in the repair after trauma.