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包公藤甲素,简称包甲素,是从草药包公藤茎中提得的新生物碱,是一个新型的拟胆碱药物,目前临床上用以治疗青光眼,其药理作用及扩大临床的应用还正在研究中。本文报导了包甲素的气液色谱分析法。将包甲素与三氟醋酐(TFAA)反应制成包甲素三氟乙酰衍生物,根据质谱数据推测,该衍生物为N-三氟乙酰-2β-三氟乙酰氧基-6β-乙酰氧基去甲茛菪烷(N-trifluroactyl-2β-triflu-roacetoxy-6β-acetoxy nortropane)。取此衍生物的正庚烷溶液在充填以涂渍了3%OV-17的Chromosorb W A W DMCS的色谱柱上进行气液色谱分析。以纯氮为载气,用电子俘获检测器(ECD)检测。实验结果表明:包甲素三氟乙酰衍生物的色谱峰面积与相应的包甲素溶液浓度在5～50μg/ml范围内呈线性关系。
Triptolide, referred to as Baogan, is a new alkaloid extracted from the herbaceous cortex cane stem, is a new type of cholinergic drugs currently used clinically to treat glaucoma, its pharmacological effects and the expansion of clinical application also In research. This article reports the gas-liquid chromatographic analysis of tampon A. According to mass spectrometry data, it is supposed that this derivative is N-trifluoroacetyl-2β-trifluoroacetoxy-6β-acetyl N-trifluroactyl-2β-triflu-roacetoxy-6β-acetoxy nortropane. A n-heptane solution of this derivative was subjected to gas-liquid chromatography on a column packed with Chromosorb W A W DMCS coated with 3% OV-17. Pure nitrogen as carrier gas, with electronic capture detector (ECD) detection. The experimental results show that the peak area of the tricyclic acetyl triflate is linear with the corresponding concentration of the tamoxifen in the range of 5 ~ 50μg / ml.