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以慢生型大豆根瘤菌(Bradyrhizobium japonicum)2-39为供试菌株,分别以不同浓度(5,10,15,20,25μg·mL~(-1))的大豆黄素、4-甲基伞形酮、金雀异黄酮、芒柄黄花素为诱导物,利用毛细管培养法对趋化的根瘤菌进行定量测定,研究不同种类和不同浓度的外源类黄酮对大豆根瘤菌趋化性的影响。选取对根瘤菌趋化性影响显著的外源类黄酮与大豆嫩丰16进行盆栽试验,进一步探究外源类黄酮对大豆结瘤效果的影响。结果表明:4种外源性类黄酮中,大豆黄素和金雀异黄酮对根瘤菌的趋化作用显著强于其它试验组,其最适作用浓度为15μg·mL~(-1)。盆栽试验结果显示:外源类黄酮大豆黄素和金雀异黄酮同时加入能够有效促进大豆结瘤。
The Bradyrhizobium japonicum 2-39 strain was used as test strain and treated with different concentrations (5, 10, 15, 20 and 25 μg · mL -1) of daidzein, 4-methyl Umbelliferone, genistein, and mangosteen were used as inducers, and the chemotactic rhizobia were quantitatively determined by capillary culture method. The effects of exogenous flavonoids of different kinds and concentrations on the chemotaxis of soybean rhizobium influences. In order to explore the effect of exogenous flavonoids on the nodulation efficiency of soybean, the exogenous flavonoids and soybean Nenfeng 16, which had a significant effect on the chemotaxis of rhizobia, were selected for pot experiment. The results showed that among four kinds of exogenous flavonoids, daidzein and genistein had stronger chemotaxis to Rhizobia than the other experimental groups, and the optimum concentration was 15μg · mL ~ (-1). The results of pot experiment showed that addition of exogenous flavonoids daidzein and genistein could effectively promote soybean nodulation.