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为研究细胞间粘附分子 1 (ICAM 1 )在孕早期的作用 ,本文观察了孕早期小鼠子宫内膜 ICAM 1蛋白水平的表达规律及细胞因子 L IF、IL 1、GM CSF和 IL 6对孕 d 4子宫内膜 ICAM 1表达的调节。方法 :1 .收集孕 1～ 8d小鼠子宫内膜 ;2 .将孕鼠随机分为实验组和对照组 ,在孕 d2～ 4分别腹腔注射不同的细胞因子或 PBS,d4收集子宫内膜。采用 Western blot方法测定子宫内膜 ICAM 1的蛋白表达水平。结果显示 :ICAM1呈时序性表达 ,孕 d1表达量较低 ,d2开始升高 ,到 d4达高峰 ,此后缓慢下降。L IF、IL1 β、IL 6、GM CSF对子宫内膜 ICAM 1的表达有促进作用。结论 :ICAM 1在围着床期表达水平较高 ,可能参与了胚胎着床。细胞因子 L IF、IL 1、IL 6和 GM CSF对其表达有促进作用。
In order to study the role of intercellular adhesion molecule-1 (ICAM 1) in early pregnancy, the expression of ICAM-1 in the endometrium of pregnant mice and the expression of cytokines L IF, IL 1, GM CSF and IL 6 Regulation of Endometrial ICAM 1 Expression in Pregnant. The pregnant mice were randomly divided into experimental group and control group, different cytokines or PBS were intraperitoneally injected at d2 ~ 4 of pregnancy, and the endometrium was collected at d4. Western blot was used to determine the protein expression of ICAM-1 in the endometrium. The results showed that the expression of ICAM1 was time-dependent, the expression level of d1 was low, d2 began to rise, peaked at d4, and then decreased slowly. L IF, IL1β, IL6 and GM CSF could promote the expression of ICAM 1 in endometrium. CONCLUSION: ICAM 1 is highly expressed in peri-implantation phase and may be involved in embryo implantation. Cytokines L IF, IL 1, IL 6 and GM CSF promote their expression.