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目的利用高通量的组织芯片技术,对胃癌组织及癌旁组织的p53、p16和环氧合酶-2 (COX-2)蛋白异常表达进行分析,探讨其相关性及临床意义。方法利用组织芯片技术结合免疫组化法检测50例胃癌组织和78例癌旁组织中p53、p16及COX-2蛋白的表达。结果p53、p16和COX-2的阳性表达率在癌旁组织中分别是19%、15%及74%；在癌组织中分别是50%、54%及94%。胃癌组织中p53、p16和COX-2蛋白表达均显著高于癌旁组织,差异具有统计学意义(P＜0．05)。p53与COX-2、p16与COX-2蛋白表达均存在相关性(P＜0．05)。当p53、COX-2表达阳性,p16阴性时；或p16、COX-2表达阳性,p53阴性时；或p53、p16和COX-2同时表达阳性时,组织芯片病理类型为癌性的概率均增加,OR值分别为11．667、30．000及18．889,p53、p16和COX-2三者存在交互作用。结论联合分析p53、p16及COX-2的表达对预测胃癌的发生和发展具有重要意义。
Objective To analyze the abnormal expression of p53, p16 and cyclooxygenase-2 (COX-2) protein in gastric cancer tissue and paracancerous tissues by high-throughput tissue microarray technique and to explore their correlation and clinical significance. Methods The expression of p53, p16 and COX-2 protein in 50 cases of gastric carcinoma and 78 cases of paracancerous tissues were detected by tissue microarray and immunohistochemistry. Results The positive expression rates of p53, p16 and COX-2 were 19%, 15% and 74% in paracancerous tissues and 50%, 54% and 94% in cancerous tissues respectively. The expressions of p53, p16 and COX-2 in gastric cancer tissues were significantly higher than those in paracancerous tissues (P <0.05). There was a correlation between p53 and COX-2, p16 and COX-2 protein expression (P <0.05). When the positive expression of p53, COX-2, p16 negative, or p16, COX-2 positive, p53 negative, or p53, p16 and COX-2 positive expression, the probability of the pathological type of cancer were increased , OR values were 11.667, 30.000 and 18.889, respectively. There was interaction between p53, p16 and COX-2. Conclusion The combined analysis of p53, p16 and COX-2 expression is of great significance in predicting the occurrence and development of gastric cancer.