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目的:观察侧脑室注射食欲素A(OXA)对大鼠胃运动的作用及作用途径。方法:选取成年雄性大鼠50只,随机分为5组,即生理盐水(NS)组、1μg组、5μg组、10μg组和20μg组。在胃窦和十二指肠植入应力传感器,记录大鼠胃肠自发性运动。通过侧脑室注射OXA,观察胃肠环形肌运动波形的变化和持续时间。选取10只大鼠随机分为2组,一组皮下注射NS+10μg OXA,另一组皮下注射阿托品+10μg OXA,观察OXA对胃肠运动的影响。同理选取10只大鼠分为假手术组+10μg OXA组,迷走神经切断组+10μg OXA组,观察OXA调控大鼠胃肠运动的作用途径。再选取10只大鼠,先注射NS再注射OXA拮抗剂SB334867,观察内源性OXA对胃肠运动的影响。结果:侧脑室注射OXA(1-20μg),大鼠消化间期胃和十二指肠III期收缩波消失,继而出现胃和十二指肠餐后不规则的收缩波。OXA对餐后胃肠运动的影响可被阿托品或切断迷走神经干所阻断(P<0.05)。中枢注射选择性OXA受体拮抗剂SB-334867(16μg),可增强胃消化间期III相收缩(P<0.05)。结论:中枢注射OXA可能经迷走神经胆碱能通路调控大鼠消化间期的胃肠运动,内源性OXA可能对大鼠消化间期胃肠运动具有抑制作用。
Objective: To observe the effect and mechanism of intracerebroventricular injection of orexin A (OXA) on gastric motility in rats. Methods: Fifty adult male rats were randomly divided into 5 groups: NS, 1μg, 5μg, 10μg and 20μg groups. Stress sensors were implanted into gastric antrum and duodenum to record spontaneous gastrointestinal motility in rats. OXA was injected into lateral ventricle to observe the change and duration of the waveform of gastrointestinal motility. Ten rats were randomly divided into two groups. One group received NS + 10μg OXA subcutaneously and the other group received subcutaneous injection of atropine + 10μg OXA to observe the effect of OXA on gastrointestinal motility. Similarly, 10 rats were divided into sham operation group + 10μg OXA group, vagotomy group + 10μg OXA group, to observe OXA regulation of gastrointestinal motility in rats. Ten rats were selected again, and NS and OXA antagonist SB334867 were injected first to observe the effect of endogenous OXA on gastrointestinal motility. Results: Intracerebroventricular injection of OXA (1-20 μg) disappeared in the intestine and duodenum of stage III contractile waves, followed by gastric and duodenal irregular post-prandial contractile waves. The effect of OXA on postprandial gastrointestinal motility was blocked by atropine or vagotomy (P <0.05). Central injection of the selective OXA receptor antagonist SB-334867 (16 μg) enhanced systolic phase III gastric contraction (P <0.05). CONCLUSION: Central injection of OXA may regulate vaginal cholinergic pathway through gastrointestinal motility during the interdigestive period. Endogenous OXA may inhibit gastrointestinal motility during the interdigestive period.