应用响应面法对猪血浆蛋白酶解制备铁螯合多肽的工艺进行优化,在单因素试验的基础上,选择pH值、温度、时间为影响因素,水解度为指标,进行三因素三水平的BoxBehnken中心组合试验设计,采用响应面法分析3个因素对响应值的影响。结果表明,制备蛋白粉最佳工艺条件为:pH值为7.7,温度为46.3℃,时间为7.4 h,在该条件下,蛋白水解度的为35.52%,水解物螯合率为79.41%。该工艺可为猪血浆蛋白开发利用提供新的思路。 Response surface methodology was used to optimize the preparation of iron-chelating peptides by proteolysis of pigs. Based on the single-factor experiments, pH, temperature and time were selected as influencing factors, and the degree of hydrolysis was used as an index. Three-factor and three-level BoxBehnken The center combination experimental design, using response surface analysis of three factors on the response value. The results showed that the optimum conditions for the preparation of protein powder were as follows: pH 7.7, temperature 46.3 ℃, time 7.4 h, under these conditions, the degree of protein hydrolysis was 35.52% and the chelating rate of hydrolyzate was 79.41%. The process can provide new ideas for the development and utilization of porcine plasma protein.