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目的 研究脊髓灰质炎病毒感染与细胞凋亡的关系及凋亡过程中bcl-2基因家族的表达特点。方法 采用人胚肺二倍体细胞(KMB17),在脊髓灰质炎病毒(Poliovirus,PV)感染后不同时间经RT-PCR等方法分析凋亡过程中bcl-2基因家族(bcl-2、bax、bak)转录水平的表达情况。结果 PV感染后诱导细胞凋亡过程中bcl-2家族中抗凋亡基因和促凋亡基因的表达均呈现趋势变化,bak和bax表达增强、bcl-2表达较弱。结论 bcl-2基因家族中抗凋亡基因和促凋亡基因表达水平的差异是脊髓灰质炎病毒诱导凋亡的机制之一。
Objective To study the relationship between poliovirus infection and apoptosis and the expression of bcl-2 gene family during apoptosis. Methods Human embryonic lung diploid cells (KMB17) were used to detect the expression of bcl-2, bax, bcl-2 and bcl-2 in the process of apoptosis by RT-PCR at different times after infection with poliovirus (PV) bak transcription level of expression. Results The expression of anti-apoptotic and pro-apoptotic genes in the bcl-2 family during the induction of apoptosis after PV infection showed a trend of change, the expression of bak and bax increased, and the expression of bcl-2 was weak. Conclusion The differences in the expression levels of anti-apoptotic and pro-apoptotic genes in the bcl-2 gene family are one of the mechanisms by which poliovirus induces apoptosis.