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目的:考察甘遂醋炙前后对正常及癌性腹水模型大鼠毒性的差异。方法:以正常及癌性腹水模型大鼠为研究对象,分组后连续7 d灌胃生、醋甘遂粉末及其提取物,各给药组按0.34 mg·g-1灌胃,空白组与模型组给予0.5%羧甲基纤维素钠。考察给药后对大鼠肝、胃、肠组织病理形态学、血清肝功能指标、氧化损伤指标的影响。结果:与空白组比较,各正常给药组大鼠肝、胃、肠组织均出现显著损伤,血清天门冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT)和碱性磷酸酶(ALP)活力显著升高,谷胱甘肽(GSH)和超氧化物歧化酶(SOD)含量显著降低,丙二醛(MDA)和乳酸脱氢酶(LDH)含量显著升高;其中生甘遂粉末作用最为显著,醋甘遂该作用显著降低。与模型组比较,各模型给药组大鼠组织损伤有所减轻,血清AST,ALT,ALP活力显著降低,GSH和SOD含量显著升高,MDA和LDH含量显著降低;其中模型生、醋粉组功效最为显著,且两组间无显著性差异。结论:甘遂醋炙后对正常动物的毒性较生甘遂低;在模型动物上,生、醋甘遂均可显著缓解模型的损伤。
OBJECTIVE: To investigate the difference of toxicities of kansui vinegar before and after Sunburn to normal and cancerous ascites rats. Methods: The normal and cancerous ascites rats were used as the research object. The rats in the ascites group were given gavage for 7 days, The model group was given 0.5% sodium carboxymethyl cellulose. The effects of administration on pathological morphology of liver, stomach and intestine, serum liver function indexes and oxidative damage indexes were investigated. Results: Compared with the blank group, the liver, stomach and intestine of the rats in each group were significantly damaged, the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activity was significantly increased, glutathione (GSH) and superoxide dismutase (SOD) content was significantly decreased, malondialdehyde (MDA) and lactate dehydrogenase (LDH) content was significantly increased; Kansui powder role is most significant, vinegar Kansui effect was significantly reduced. Compared with the model group, the rats in each model group had lessened tissue injury, AST, ALT, ALP, GSH and SOD, and MDA and LDH in the model group decreased significantly Efficacy of the most significant, and no significant difference between the two groups. Conclusion: The toxicity of kansui vinegar to normal animals is lower than that of hygromycin. In model animals, the injury of model and model group can be significantly alleviated.